Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/53685
DC Field | Value | |
---|---|---|
dc.title | THE ANALYSIS OF THE WNT-RYK SIGNALING PATHWAY IN C. ELEGANS | |
dc.contributor.author | POH WOON CHENG | |
dc.date.accessioned | 2014-05-31T18:02:20Z | |
dc.date.available | 2014-05-31T18:02:20Z | |
dc.date.issued | 2014-01-24 | |
dc.identifier.citation | POH WOON CHENG (2014-01-24). THE ANALYSIS OF THE WNT-RYK SIGNALING PATHWAY IN C. ELEGANS. ScholarBank@NUS Repository. | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/53685 | |
dc.description.abstract | Wnt signaling is well known to regulate various cellular processes during development. Wnt ligands can bind to Fz, Ryk or Ror receptors. In this study, we focused on Wnt-Ryk signaling. Ryk is a single-pass transmembrane receptor, which consists of an extracellular domain (ECD), a transmembrane region (TM) and the intracellular domain (ICD). In C. elegans, lin-18 encodes the Ryk ortholog. We showed that expression of LIN-18ECD-TM is sufficient to rescue the lin-18 loss-of-function phenotype, consistent with previous finding. In contrast, overexpression of LIN-18ICD enhanced the lin-18 loss-of-function phenotype. Like mammalian RykICD, LIN-18ICD tends to localize to the nucleus. We found that the CAM-1/Ror signaling pathway and PAR-5/14-3-3 proteins may regulate LIN-18ICD nuclear localization. In addition, we also studied the role of lipid modification in Wnt signaling. We found that LIN-17/Fz and CAM-1/Ror signaling, but not LIN-18/Ryk signaling, require Porcupine mediated lipid modification of Wnt ligands. | |
dc.language.iso | en | |
dc.subject | Wnt, Ryk, Wnt lipid modification, Wntless, Porcupine, C. elegans | |
dc.type | Thesis | |
dc.contributor.department | BIOCHEMISTRY | |
dc.contributor.supervisor | TAKAO INOUE | |
dc.description.degree | Ph.D | |
dc.description.degreeconferred | DOCTOR OF PHILOSOPHY | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Ph.D Theses (Open) |
Show simple item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
PohWC.pdf | 2.09 MB | Adobe PDF | OPEN | None | View/Download |
Google ScholarTM
Check
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.