Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0024339
Title: Meibum lipid composition in Asians with dry eye disease
Authors: Lam, S.M.
Tong, L. 
Yong, S.S.
Li, B.
Chaurasia, S.S.
Shui, G. 
Wenk, M.R.
Issue Date: 17-Oct-2011
Source: Lam, S.M., Tong, L., Yong, S.S., Li, B., Chaurasia, S.S., Shui, G., Wenk, M.R. (2011-10-17). Meibum lipid composition in Asians with dry eye disease. PLoS ONE 6 (10) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0024339
Abstract: Background: Previous lipidomic analyses of the human meibum had largely focused on individuals from non-Asian populations, despite the higher prevalence of dysfunctional tear syndrome (DTS) observed across Asia. Information pertaining to the alterations in lipid profiles in relation to DTS onset and progression is also lacking and warrants comprehensive experimental analysis. Methodologies/Principal Findings: We examined the meibum lipidome of 27 DTS patients and 10 control subjects for a total of 256 lipid species from 12 major lipid classes, including cholesteryl ester (CE), wax ester (WE), triacylglyceride (TAG), (O-acyl)-ω-hydroxy fatty acid (OAHFA), glycerophospholipids (phosphatidylcholine, PC; phosphatidylethanolamine, PE; phosphatidylinositol, PI; phosphatidylglycerol, PG) and sphingolipids (sphingomyelin, SM; ceramide, Cer; glucosylceramide, GluCer; dihexosylceramide, DihexCer). Neutral lipids were analysed using high-performance liquid-chromatography coupled with mass spectrometry (HPLC/MS) and tandem mass spectrometry (MS/MS) was used for the qualitative and quantitative analysis of polar lipid species. DTS patients were classified into three severity groups (i.e. mild, moderate and severe) based on the ocular surface disease index (OSDI). A significantly lower level of TAG (p≤0.05) was observed in patients under the moderate category compared to the mild category. Notably, a number of OAHFA species displayed consistently decreasing levels that correlate with increasing disease severity. An attempt was also made to investigate the changes in meibum lipid profiles of DTS patients compared to normal individuals classified based on OSDI score. Several unsaturated TAG and PC species were found at significantly higher levels (p≤0.05) in patients than controls. Conclusion: The current study presents, for the first time, a comprehensive lipidome of meibum from individuals of an Asian ethnicity, which can potentially offer new insights into the higher prevalence of DTS observed amongst Asian populations. This study also represents an attempt towards identification of lipid species in meibum which could serve as marker for DTS. © 2011 Lam et al.
Source Title: PLoS ONE
URI: http://scholarbank.nus.edu.sg/handle/10635/53459
ISSN: 19326203
DOI: 10.1371/journal.pone.0024339
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