Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jviromet.2004.01.002
Title: In situ hybridization of a marine fish virus, Singapore grouper iridovirus with a nucleic acid probe of major capsid protein
Authors: Huang, C. 
Zhang, X.
Gin, K.Y.H.
Qin, Q.W. 
Keywords: DNA probe
Grouper
In situ hybridization
Iridovirus
Issue Date: May-2004
Source: Huang, C., Zhang, X., Gin, K.Y.H., Qin, Q.W. (2004-05). In situ hybridization of a marine fish virus, Singapore grouper iridovirus with a nucleic acid probe of major capsid protein. Journal of Virological Methods 117 (2) : 123-128. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jviromet.2004.01.002
Abstract: A DNA probe of 531 base pairs for Singapore grouper iridovirus (SGIV) was generated by polymerase chain reaction and labeled with nonradioactive digoxigenin. An in situ hybridization based method was developed to detect SGIV in formalin-fixed tissues from maricultured Malabar grouper, Epinephelus malabaricus Bloch and Schneider. The in situ hybridization detected SGIV in the kidney, spleen, liver, intestine, stomach and gills from naturally infected fish. Strong hybridization signals were obtained from the kidney and spleen tissues, while intermediate intensity signals were observed in the intestine and liver tissues. The weakest signals were obtained from the stomach and gills. The signals were located specifically within epithelial, endothelial and sub-endothelial hypertrophic cells in all tested tissues. The in situ hybridization procedure will provide an important diagnostic tool to complement histopathological methods, and contribute to epidemiological studies on the origin and distribution of iridovirus in mariculture. © 2004 Elsevier B.V. All rights reserved.
Source Title: Journal of Virological Methods
URI: http://scholarbank.nus.edu.sg/handle/10635/53448
ISSN: 01660934
DOI: 10.1016/j.jviromet.2004.01.002
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