Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/53437
Title: Functional complementation of the malvolio mutation in the taste pathway of Drosophila melanogaster by the human natural resistance-associated macrophage protein 1 (Nramp-1)
Authors: D'Souza, J.
Cheah, P.Y. 
Gros, P.
Chia, W. 
Rodrigues, V.
Keywords: Drosophila melanogaster
Feeding preference assay
Malvolio
Natural resistance-associated macrophage protein
Nramp-1
Taste behaviour
Issue Date: Jul-1999
Source: D'Souza, J.,Cheah, P.Y.,Gros, P.,Chia, W.,Rodrigues, V. (1999-07). Functional complementation of the malvolio mutation in the taste pathway of Drosophila melanogaster by the human natural resistance-associated macrophage protein 1 (Nramp-1). Journal of Experimental Biology 202 (14) : 1909-1915. ScholarBank@NUS Repository.
Abstract: The malvolio (mvl) gene of Drosophila melanogaster encodes a protein with a high degree of homology to natural resistance-associated macrophage proteins (Nramps). This family of integral membrane proteins, many of which appear to function as cation transporters, is remarkably conserved in several phylogenetically distinct species. In Drosophila melanogaster, the protein Mvl is expressed in macrophages and in differentiated neurons; loss-of-function mutations lead to defects in gustatory behaviour. The human Nramp-1 protein was expressed in Drosophila melanogaster using the hsp70 promoter. Overexpression in normal animals does not lead to any alterations in their behaviour or physiology. In mutants, however, ubiquitous expression of human Nramp-1 can totally rescue the taste defect. This finding that Nramp-1 can complement the taste defect in mvl mutants provides a potent means of exploiting behavioural genetics to dissect the function of Nramp-1 and to identify other molecules involved with this transport system.
Source Title: Journal of Experimental Biology
URI: http://scholarbank.nus.edu.sg/handle/10635/53437
ISSN: 00220949
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

43
checked on Dec 8, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.