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https://scholarbank.nus.edu.sg/handle/10635/53272
Title: | Analysis of recombinant human tumor necrosis factor beta by capillary electrophoresis | Authors: | Yao, Y.J. Loh, K.C. Chung, M.C.M. Li, S.F.Y. |
Keywords: | capillary electrophoresis recombinant-DNA technology tumor necrosis factor |
Issue Date: | 1995 | Citation: | Yao, Y.J.,Loh, K.C.,Chung, M.C.M.,Li, S.F.Y. (1995). Analysis of recombinant human tumor necrosis factor beta by capillary electrophoresis. Electrophoresis 16 (4) : 647-653. ScholarBank@NUS Repository. | Abstract: | Capillary electrophoresis was used for the analysis of recombinnt tumor necrosis factor beta (rhTNF-β). Reproducible separation of the protein could be achieved in Polybrene-coated capillaries. Optimum conditions for separation were achieved with a pH 4.5 sodium acetate buffer. Besides Polybrene-coated capillaries, other commercially available column and column-coating reagents were also examined for the separation of rhTNF-β. Capillary electrophoresis was found to be a potentially useful method for product analysis and process monitoring in recombinant-DNA technology. | Source Title: | Electrophoresis | URI: | http://scholarbank.nus.edu.sg/handle/10635/53272 | ISSN: | 01730835 |
Appears in Collections: | Staff Publications |
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