Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/53263
Title: Zebrafish tbx-c functions during formation of midline structures
Authors: Dheen, T.
Sleptsova-Friedrich, I.
Xu, Y.
Clark, M.
Lehrach, H.
Gong, Z. 
Korzh, V. 
Keywords: Animal cap
Chordoneural hinge
Dominant-negative
flh
islet1
Motor neuron
myoD
Notochord
ntl
Overexpression
shh
Somite
spt
T-box gene
tbx6
Issue Date: Jun-1999
Source: Dheen, T.,Sleptsova-Friedrich, I.,Xu, Y.,Clark, M.,Lehrach, H.,Gong, Z.,Korzh, V. (1999-06). Zebrafish tbx-c functions during formation of midline structures. Development 126 (12) : 2703-2713. ScholarBank@NUS Repository.
Abstract: Several genes containing the conserved T-box region in invertebrates and vertebrates have been reported recently. Here, we describe three novel members of the T-box gene family in zebrafish. One of these genes, tbx-c, is studied in detail. It is expressed in the axial mesoderm, notably, in the notochordal precursor cells immediately before formation of the notochord and in the chordoneural hinge of the tail bud, after the notochord is formed. In addition, its expression is detected in the ventral forebrain, sensory neurons, fin buds and excretory system. The expression pattern of tbx-c differs from that of the other two related genes, tbx-a and tbx-b. The developmental role of tbx-c has been analysed by overexpression of the full-length tbx-c mRNA and a truncated form of tbx-c mRNA, which encodes the dominant-negative Tbx-c. Overexpression of tbx-c causes expansion of the midline mesoderm and formation of ectopic midline structures at the expense of lateral mesodermal cells. In dominant-negative experiments, the midline mesoderm is reduced with the expansion of lateral mesoderm to the midline. These results suggest that tbx-c plays a role in formation of the midline mesoderm, particularly, the notochord. Moreover, modulation of tbx-c activity alters the development of primary motor neurons. Results of in vitro analysis in zebrafish animal caps suggest that tbx-c acts downstream of early mesodermal inducers (activin and ntl) and reveal an autoregulatory feedback loop between nlt and tbx-c. These data and analysis of midline (ntl(-/-) and flh(-/-)) and lateral mesoderm (spt(-/-)) mutants suggest that tbx-c may function during formation of the notochord.
Source Title: Development
URI: http://scholarbank.nus.edu.sg/handle/10635/53263
ISSN: 09501991
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

44
checked on Dec 15, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.