Please use this identifier to cite or link to this item: https://doi.org/10.1016/0166-0934(96)02051-4
Title: Study of the three-dimensional images of potyvirus-induced cytoplasmic inclusions using confocal laser scanning microscopy
Authors: Lim, T.M. 
Chng, C.G. 
Wong, S.M. 
Keywords: Confocal laser scanning microscopy
Cytoplasmic inclusion bodies
Potyviruses
Three-dimensional structure
Zucchini yellow mosaic virus
Issue Date: Jul-1996
Source: Lim, T.M., Chng, C.G., Wong, S.M. (1996-07). Study of the three-dimensional images of potyvirus-induced cytoplasmic inclusions using confocal laser scanning microscopy. Journal of Virological Methods 60 (2) : 139-145. ScholarBank@NUS Repository. https://doi.org/10.1016/0166-0934(96)02051-4
Abstract: The three-dimensional structure of the cytoplasmic inclusions in leaves of Cucurbita pepo L. infected with a Singapore isolate of zucchini yellow mosaic virus (ZYMV-S) was studied using a confocal laser scanning microscope. The cytoplasmic inclusions were found to be linear filamentous structures that varied in thickness and length. The formation of the inclusions probably begins when finely-pointed linear filamentous structures aggregate into bundles forming flat parallel arrays, or diverge into triangular shaped inclusions. Some cytoplasmic inclusions appeared as twisted tubules with empty central cores. The average length and width of the inclusions ranged from 9.4 ± 0.3 to 20.1 ± 0.4 μm and from 2.1 ± 0.1 to 3.7 ± 0.1 μm, respectively. Confocal laser scanning microscopy is useful for the in situ exploration of three-dimensional structures of cytoplasmic or nuclear inclusions in plant virus infected cells.
Source Title: Journal of Virological Methods
URI: http://scholarbank.nus.edu.sg/handle/10635/53196
ISSN: 01660934
DOI: 10.1016/0166-0934(96)02051-4
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