Please use this identifier to cite or link to this item: https://doi.org/10.1007/s00705-002-0936-x
Title: Nodavirus infection in freshwater ornamental fish, guppy, Poicelia reticulata - Comparative characterization and pathogenicity studies
Authors: Hegde, A. 
Teh, H.C.
Lam, T.J. 
Sin, Y.M. 
Issue Date: 1-Mar-2003
Source: Hegde, A., Teh, H.C., Lam, T.J., Sin, Y.M. (2003-03-01). Nodavirus infection in freshwater ornamental fish, guppy, Poicelia reticulata - Comparative characterization and pathogenicity studies. Archives of Virology 148 (3) : 575-586. ScholarBank@NUS Repository. https://doi.org/10.1007/s00705-002-0936-x
Abstract: Biochemical, genomic and serological studies were carried out to characterize a virus obtained from diseased guppy, Poicelia reticulata. The SDS-PAGE analysis of CsCl purified virus showed two distinct bands with molecular weight of 42 kDa and 110 kDa. A 1367 nucleotide region of the coat protein gene was sequenced, which includes one full open reading frame of 1017 nucleotides and a region of 350 nucleotides at the 3′end. The nucleotide identity of this strain with the nodavirus isolated from Epinephelus tauvina (Singapore strain) is 98% and with other strains of fish nodaviruses the identity is more than 75%. Western blot analysis using rabbit antisera raised against the nodavirus from marine fish, E. tauvina confirmed its antigenic similarity to the marine nodavirus isolate. Asymptomatic infection in guppy fry was observed following experimental infection with this virus and the marine nodavirus isolate (Singapore strain) implying the spread of virus from marine fish to freshwater fish. This report forms the first description of a nodavirus infection in freshwater fish.
Source Title: Archives of Virology
URI: http://scholarbank.nus.edu.sg/handle/10635/53056
ISSN: 03048608
DOI: 10.1007/s00705-002-0936-x
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

53
checked on Dec 13, 2017

WEB OF SCIENCETM
Citations

40
checked on Dec 13, 2017

Page view(s)

44
checked on Dec 9, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.