Please use this identifier to cite or link to this item: https://doi.org/10.1073/pnas.1200817109
Title: Neural stem cell specific fluorescent chemical probe binding to FABP7
Authors: Yun, S.-W.
Leong, C.
Zhai, D.
Tan, Y.L.
Lim, L.
Bi, X.
Lee, J.-J.
Kim, H.J. 
Kang, N.-Y.
Ng, S.H.
Stanton, L.W.
Chang, Y.-T. 
Keywords: BLBP
DOFL
High throughput screening
Mass spectrometry
Radial glia
Issue Date: 26-Jun-2012
Source: Yun, S.-W., Leong, C., Zhai, D., Tan, Y.L., Lim, L., Bi, X., Lee, J.-J., Kim, H.J., Kang, N.-Y., Ng, S.H., Stanton, L.W., Chang, Y.-T. (2012-06-26). Neural stem cell specific fluorescent chemical probe binding to FABP7. Proceedings of the National Academy of Sciences of the United States of America 109 (26) : 10214-10217. ScholarBank@NUS Repository. https://doi.org/10.1073/pnas.1200817109
Abstract: Fluorescent small molecules have become indispensable tools for biomedical research along with the rapidly developing optical imaging technology. We report here a neural stem cell specific borondipyrromethane (BODIPY) derivative compound of designation red 3 (CDr3), developed through a high throughput/content screening of in-house generated diversity oriented fluorescence library in stem cells at different developmental stages. This novel compound specifically detects living neural stem cells of both human and mouse origin. Furthermore, we identified its binding target by proteomic analysis as fatty acid binding protein 7 (FABP7), also known as brain lipid binding protein) which is highly expressed in neural stem cells and localized in the cytoplasm. CDr3 will be a valuable chemical tool in the study and applications of neural stem cells.
Source Title: Proceedings of the National Academy of Sciences of the United States of America
URI: http://scholarbank.nus.edu.sg/handle/10635/53053
ISSN: 00278424
DOI: 10.1073/pnas.1200817109
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