Please use this identifier to cite or link to this item: https://doi.org/10.1117/1.3094947
Title: Line scan fluorescence correlation spectroscopy for three-dimensional microfluidic flow velocity measurements
Authors: Pan, X.
Shi, X. 
Korzh, V.
Yu, H. 
Wohland, T. 
Keywords: 3-D velocity measurement
flow direction
fluorescence correlation spectroscopy
line scan
microfluidics
Issue Date: 2009
Source: Pan, X., Shi, X., Korzh, V., Yu, H., Wohland, T. (2009). Line scan fluorescence correlation spectroscopy for three-dimensional microfluidic flow velocity measurements. Journal of Biomedical Optics 14 (2) : -. ScholarBank@NUS Repository. https://doi.org/10.1117/1.3094947
Abstract: The flow direction of microfluidics in biological applications is not limited to two dimensions, but often extends to three dimensions. Currently there are optical methods available for the measurement of 3-D microfluidic flow vectors, but with low spatial resolution. Line scan fluorescence correlation spectroscopy (FCS) was proposed to determine flow directions in 2-D within microchannels and small blood vessels in our previous work. Importantly, its spatial resolution was demonstrated to be as good as 0.5μm. In this work, we extend line scan FCS to the third dimension for the characterization of 3-D flow velocity vectors. The spatial resolution is close to the diffraction limit using a scan length of 0.5μm in all three dimensions. The feasibility of line scan FCS for 3-D microfluidic flow is verified by measurements in microchannels and small blood vessels of zebrafish embryos. © 2009 Society of Photo-Optical Instrumentation Engineers.
Source Title: Journal of Biomedical Optics
URI: http://scholarbank.nus.edu.sg/handle/10635/53011
ISSN: 10833668
DOI: 10.1117/1.3094947
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