Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.molcel.2013.04.030
Title: Genome-wide Kinase-Chromatin Interactions Reveal the Regulatory Network of ERK Signaling in Human Embryonic Stem Cells
Authors: Göke, J.
Chan, Y.-S.
Yan, J. 
Vingron, M.
Ng, H.-H. 
Issue Date: 27-Jun-2013
Source: Göke, J., Chan, Y.-S., Yan, J., Vingron, M., Ng, H.-H. (2013-06-27). Genome-wide Kinase-Chromatin Interactions Reveal the Regulatory Network of ERK Signaling in Human Embryonic Stem Cells. Molecular Cell 50 (6) : 844-855. ScholarBank@NUS Repository. https://doi.org/10.1016/j.molcel.2013.04.030
Abstract: The extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase signal-transduction cascade is one of the key pathways regulating proliferation and differentiation in development and disease. ERK signaling is required for human embryonic stem cells' (hESCs') self-renewing property. Here, we studied the convergence of the ERK signaling cascade at the DNA by mapping genome-wide kinase-chromatin interactions for ERK2 in hESCs. We observed that ERK2 binding occurs near noncoding genes and histone, cell-cycle, metabolism, and pluripotency-associated genes. We find that the transcription factor ELK1 is essential in hESCs and that ERK2 co-occupies promoters bound by ELK1. Strikingly, promoters bound by ELK1 without ERK2 are occupied by Polycomb group proteins that repress genes involved in lineage commitment. In summary, we propose a model wherein extracellular-signaling-stimulated proliferation and intrinsic repression of differentiation are integrated to maintain the identity of hESCs. © 2013 Elsevier Inc.
Source Title: Molecular Cell
URI: http://scholarbank.nus.edu.sg/handle/10635/52957
ISSN: 10972765
DOI: 10.1016/j.molcel.2013.04.030
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

41
checked on Dec 6, 2017

WEB OF SCIENCETM
Citations

40
checked on Nov 21, 2017

Page view(s)

48
checked on Dec 10, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.