Please use this identifier to cite or link to this item: https://doi.org/10.1039/c1lc20351g
Title: Fish and chips: A microfluidic perfusion platform for monitoring zebrafish development
Authors: Choudhury, D.
Van Noort, D.
Iliescu, C.
Zheng, B.
Poon, K.-L.
Korzh, S. 
Korzh, V. 
Yu, H. 
Issue Date: 7-Mar-2012
Source: Choudhury, D.,Van Noort, D.,Iliescu, C.,Zheng, B.,Poon, K.-L.,Korzh, S.,Korzh, V.,Yu, H. (2012-03-07). Fish and chips: A microfluidic perfusion platform for monitoring zebrafish development. Lab on a Chip - Miniaturisation for Chemistry and Biology 12 (5) : 892-900. ScholarBank@NUS Repository. https://doi.org/10.1039/c1lc20351g
Abstract: We have developed a multi-channel microfluidic perfusion platform for culturing zebrafish embryos and capturing live images of various tissues and organs inside the embryo. The Fish and Chips was micro-fabricated in silicon and glass for reproducibility and accuracy of the microfluidic structure. The microfluidic platform consists of three parts: a microfluidic gradient generator, a row of eight fish tanks, in which the fish embryos are individually placed, and eight output channels. The fluidic gradient generator supports dose-dependent drug and chemical studies. A unique perfusion system ensures a uniform and constant flow of media to the fish tank while the wastes are efficiently removed. The fish tanks restrict the embryo movements, except rotationally, for live imaging of internal tissues and organs. The embryos showed developmental abnormalities under the influence of the drug valproic acid (VPA). © 2012 The Royal Society of Chemistry.
Source Title: Lab on a Chip - Miniaturisation for Chemistry and Biology
URI: http://scholarbank.nus.edu.sg/handle/10635/52941
ISSN: 14730197
DOI: 10.1039/c1lc20351g
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

51
checked on Dec 12, 2017

Page view(s)

68
checked on Dec 14, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.