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Title: Elucidating the function of an ancient NF-κB p100 homologue, CrRelish, in antibacterial defense
Authors: Ze, H.F.
Xiao, W.W. 
Lu, J. 
Ho, B.
Jeak, L.D. 
Issue Date: Feb-2008
Citation: Ze, H.F., Xiao, W.W., Lu, J., Ho, B., Jeak, L.D. (2008-02). Elucidating the function of an ancient NF-κB p100 homologue, CrRelish, in antibacterial defense. Infection and Immunity 76 (2) : 664-670. ScholarBank@NUS Repository.
Abstract: The family of NF-κB transcription factors essentially regulates immune-related gene expression. Recently, we isolated and characterized the classical NF-κB/inhibitor κB (IκB) homologues from a "living fossil," the horseshoe crab, Carcinoscorpius rotundicauda. Interestingly, this ancient species also harbors another class I NF-κB p100 homologue, C. rotundicauda Relish (CrRelish). Similar to Drosophila Relish and the mammalian p100, CrRelish contains both the Rel-homology domains (RHD) and the IκB-like domain. In this study, we found that the RHD of CrRelish can recognize horseshoe crab and human κB response elements and activate the downstream reporter in vitro, thereby suggesting the evolutionary conservation of this molecule. Pseudomonas aeruginosa infection transcriptionally upregulates CrRelish, which exhibits a dynamic protein profile over the time course of infection. Surprisingly, secondary infection reinduced an upsurge in CrRelish protein expression to a level which overrode the protein degradation at 12 h postinfection. These observations strongly suggest the involvement of CrRelish in antibacterial defense. Secondary infection causes (i) the maintenance of a favorable expression-competent sequence context of the CrRelish gene and/or (ii) the derepression or stabilization of the CrRelish transcript resulting from the primary infection to enable the more rapid expression and accumulation of the CrRelish protein, reflecting apparent signal/immune priming in a repeated infection. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
Source Title: Infection and Immunity
ISSN: 00199567
DOI: 10.1128/IAI.00948-07
Appears in Collections:Staff Publications

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