Please use this identifier to cite or link to this item:
|Title:||Electron multiplying charge-coupled device camera based fluorescence correlation spectroscopy|
|Authors:||Kannan, B. |
|Source:||Kannan, B., Har, J.Y., Liu, P., Maruyama, I., Ding, J.L., Wohland, T. (2006-05-15). Electron multiplying charge-coupled device camera based fluorescence correlation spectroscopy. Analytical Chemistry 78 (10) : 3444-3451. ScholarBank@NUS Repository. https://doi.org/10.1021/ac0600959|
|Abstract:||A fluorescence correlation spectroscopy (FCS) setup is built with an electron multiplying charge-coupled device camera. Although the instrument has a limited time resolution of 4 ms, compared to 0.1-0.2 μs for common instruments using avalanche photodiodes, it allows multiplexing of FCS measurements, has a software-adjustable pinhole after data collection, performs flow speed as well as flow direction measurements in microchannels and could be used to do spectral FCS. Measurements are performed on fluorescent dyes and polystyrene beads in high-viscosity media and on epidermal growth factor receptors in Chinese hamster ovary cells. Using real measurements on single spots, multiplexing of focal spots and detection elements are simulated and the results are discussed. © 2006 American Chemical Society.|
|Source Title:||Analytical Chemistry|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Dec 13, 2017
WEB OF SCIENCETM
checked on Nov 14, 2017
checked on Dec 16, 2017
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.