Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bios.2006.06.005
Title: Bead-based microfluidic immunoassays: The next generation
Authors: Lim, C.T. 
Zhang, Y. 
Keywords: Bead-based assays
Encoded microbeads
Immunoassays
Microfluidics
Issue Date: 15-Feb-2007
Source: Lim, C.T., Zhang, Y. (2007-02-15). Bead-based microfluidic immunoassays: The next generation. Biosensors and Bioelectronics 22 (7) : 1197-1204. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bios.2006.06.005
Abstract: Microfluidic devices possess many advantages like high throughput, short analysis time, small volume and high sensitivity that fulfill all the important criteria of an immunoassay used for clinical diagnoses, environmental analyses and biochemical studies. These devices can be made from a few different materials, with polymers presently emerging as the most popular choice. Other than being optically clear, non-toxic and cheap, polymers can also be easily fabricated with a variety of techniques. In addition, there are many polymer surface modification methods available to improve the efficiency of these devices. Unfortunately, current microfluidic immunoassays have limited multiplexing capability compared to flow cytometric assays. Flow cytometry employ the use of encoded microbeads in contrast with normal or paramagnetic microbeads applied in current microfluidic devices. The encoded microbead is the key in providing multiplexing capability to the assay by allowing multi-analyte analysis. Using several unique sets of code, different analytes can be detected in a single assay by tracing the identity of individual beads. The same principle could be applied to microfluidic immunoassays in order to retain all the advantages of a fluidic device and significantly improve multiplexing capability. © 2006 Elsevier B.V. All rights reserved.
Source Title: Biosensors and Bioelectronics
URI: http://scholarbank.nus.edu.sg/handle/10635/52581
ISSN: 09565663
DOI: 10.1016/j.bios.2006.06.005
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

176
checked on Dec 5, 2017

WEB OF SCIENCETM
Citations

164
checked on Nov 15, 2017

Page view(s)

51
checked on Dec 9, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.