Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/51963
Title: FUNCTIONAL CHARACTERIZATION OF HISTONE H2A VARIANTS IN ARABIDOPSIS THALIANA
Authors: YELAGANDULA RAMESH
Keywords: Histone H2A variants, Chromatin structure, Heterochromatin, DNA methylation, Arabidopsis, H2A Genome profiles
Issue Date: 9-Dec-2013
Source: YELAGANDULA RAMESH (2013-12-09). FUNCTIONAL CHARACTERIZATION OF HISTONE H2A VARIANTS IN ARABIDOPSIS THALIANA. ScholarBank@NUS Repository.
Abstract: Histone variants play crucial roles in gene expression, genome integrity and chromosome segregation. However, to what extent histone variants control chromatin architecture remains largely unknown. We report genome-wide profiles of all four types of H2A variants in Arabidopsis and identify that the previously uncharacterized histone variant H2A.W specifically associates with heterochromatin. Genetic analyses show that H2A.W acts in synergy with the heterochromatic marks H3K9me2 and DNA methylation to maintain genome integrity. In vitro, H2A.W enhances chromatin condensation through a higher propensity to promote fiber-to-fiber interactions via its conserved C-terminal motif. In vivo, elimination of H2A.W causes decondensation of heterochromatin and conversely, ectopic expression of H2A.W promotes heterochromatin condensation. These results demonstrate that H2A.W plays critical roles in heterochromatin by promoting higher order chromatin condensation. Since motifs similar to H2A.W C-terminal motif are present in other histone variants in other organisms, our findings impact our understanding of heterochromatin condensation in eukaryotes.
URI: http://scholarbank.nus.edu.sg/handle/10635/51963
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