Please use this identifier to cite or link to this item: https://doi.org/10.1080/10611860802582442
Title: Critical parameters in the pegylation of gold nanoshells for biomedical applications: An in vitro macrophage study
Authors: Kah, J.C.Y. 
Wong, K.Y.
Neoh, K.G. 
Song, J.H.
Fu, J.W.P.
Mhaisalkar, S.
Olivo, M. 
Sheppard, C.J.R. 
Keywords: Gold nanoshells
Macrophage uptake
Pegylation
Poly(ethylene glycol)
RES clearance
Reticuloendothelial system
Surface density
Issue Date: Apr-2009
Source: Kah, J.C.Y., Wong, K.Y., Neoh, K.G., Song, J.H., Fu, J.W.P., Mhaisalkar, S., Olivo, M., Sheppard, C.J.R. (2009-04). Critical parameters in the pegylation of gold nanoshells for biomedical applications: An in vitro macrophage study. Journal of Drug Targeting 17 (3) : 181-193. ScholarBank@NUS Repository. https://doi.org/10.1080/10611860802582442
Abstract: Pegylation of gold nanoshells provides an effective means to reduce their reticuloendothelial system (RES) clearance in body. In this study, we perform a parametric investigation on the factors that would affect the macrophage uptake of gold nanoshells with the aim to optimize their pegylation and minimize their macrophage uptake. We synthesized and pegylated the gold nanoshells using methoxy-poly(ethylene glycol)-thiol and employed an in vitro macrophage assay to examine the effect of surface density of poly(ethylene glycol) (PEG), chain length of the PEG, and size of the gold nanoshells on their macrophage uptake. We have shown that a saturated surface density would minimize macrophage uptake, which could be obtained by experimental titration-based Ellman's reagent. Our results suggest that the chain length of PEG and size of gold nanoshells influence the surface density of PEG. We have also shown that PEG with molecular weight of around 2000 Da and a size range larger than 186 nm would be appropriate for facilitating a high surface density. Our in vitro macrophage system thus provides a good model to accurately predict the RES response to different pegylation parameters. © 2009 Informa UK Ltd.
Source Title: Journal of Drug Targeting
URI: http://scholarbank.nus.edu.sg/handle/10635/51768
ISSN: 1061186X
DOI: 10.1080/10611860802582442
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

64
checked on Dec 13, 2017

WEB OF SCIENCETM
Citations

56
checked on Nov 15, 2017

Page view(s)

46
checked on Dec 9, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.