Please use this identifier to cite or link to this item:
|Title:||Critical parameters in the pegylation of gold nanoshells for biomedical applications: An in vitro macrophage study|
|Authors:||Kah, J.C.Y. |
|Source:||Kah, J.C.Y., Wong, K.Y., Neoh, K.G., Song, J.H., Fu, J.W.P., Mhaisalkar, S., Olivo, M., Sheppard, C.J.R. (2009-04). Critical parameters in the pegylation of gold nanoshells for biomedical applications: An in vitro macrophage study. Journal of Drug Targeting 17 (3) : 181-193. ScholarBank@NUS Repository. https://doi.org/10.1080/10611860802582442|
|Abstract:||Pegylation of gold nanoshells provides an effective means to reduce their reticuloendothelial system (RES) clearance in body. In this study, we perform a parametric investigation on the factors that would affect the macrophage uptake of gold nanoshells with the aim to optimize their pegylation and minimize their macrophage uptake. We synthesized and pegylated the gold nanoshells using methoxy-poly(ethylene glycol)-thiol and employed an in vitro macrophage assay to examine the effect of surface density of poly(ethylene glycol) (PEG), chain length of the PEG, and size of the gold nanoshells on their macrophage uptake. We have shown that a saturated surface density would minimize macrophage uptake, which could be obtained by experimental titration-based Ellman's reagent. Our results suggest that the chain length of PEG and size of gold nanoshells influence the surface density of PEG. We have also shown that PEG with molecular weight of around 2000 Da and a size range larger than 186 nm would be appropriate for facilitating a high surface density. Our in vitro macrophage system thus provides a good model to accurately predict the RES response to different pegylation parameters. © 2009 Informa UK Ltd.|
|Source Title:||Journal of Drug Targeting|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Mar 8, 2018
WEB OF SCIENCETM
checked on Feb 13, 2018
checked on Mar 12, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.