Please use this identifier to cite or link to this item:
|Title:||ATF5, a possible regulator of osteogenic differentiation in human adipose-derived stem cells|
|Authors:||Leong, D.T. |
|Keywords:||Adipose derived stem cells|
|Citation:||Leong, D.T., Abraham, M.C., Gupta, A., Lim, T.-C., Chew, F.T., Hutmacher, D.W. (2012-08). ATF5, a possible regulator of osteogenic differentiation in human adipose-derived stem cells. Journal of Cellular Biochemistry 113 (8) : 2744-2753. ScholarBank@NUS Repository. https://doi.org/10.1002/jcb.24150|
|Abstract:||The regulatory pathways involved in maintaining the pluripotency of embryonic stem cells are partially known, whereas the regulatory pathways governing adult stem cells and their "stem-ness" are characterized to an even lesser extent. We, therefore, screened the transcriptome profiles of 20 osteogenically induced adult human adipose-derived stem cell (ADSC) populations and investigated for putative transcription factors that could regulate the osteogenic differentiation of these ADSC. We studied a subgroup of donors' samples that had a disparate osteogenic response transcriptome from that of induced human fetal osteoblasts and the rest of the induced human ADSC samples. From our statistical analysis, we found activating transcription factor 5 (ATF5) to be significantly and consistently down-regulated in a randomized time-course study of osteogenically differentiated adipose-derived stem cells from human donor samples. Knockdown of ATF5 with siRNA showed an increased sensitivity to osteogenic induction. This evidence suggests a role for ATF5 in the regulation of osteogenic differentiation in adipose-derived stem cells. To our knowledge, this is the first report that indicates a novel role of transcription factors in regulating osteogenic differentiation in adult or tissue specific stem cells. © 2012 Wiley Periodicals, Inc.|
|Source Title:||Journal of Cellular Biochemistry|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jul 14, 2018
WEB OF SCIENCETM
checked on Jun 19, 2018
checked on May 25, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.