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Title: ATF5, a possible regulator of osteogenic differentiation in human adipose-derived stem cells
Authors: Leong, D.T. 
Abraham, M.C.
Gupta, A.
Lim, T.-C.
Chew, F.T. 
Hutmacher, D.W.
Keywords: Adipose derived stem cells
Osteogenic differentiation
Issue Date: Aug-2012
Source: Leong, D.T., Abraham, M.C., Gupta, A., Lim, T.-C., Chew, F.T., Hutmacher, D.W. (2012-08). ATF5, a possible regulator of osteogenic differentiation in human adipose-derived stem cells. Journal of Cellular Biochemistry 113 (8) : 2744-2753. ScholarBank@NUS Repository.
Abstract: The regulatory pathways involved in maintaining the pluripotency of embryonic stem cells are partially known, whereas the regulatory pathways governing adult stem cells and their "stem-ness" are characterized to an even lesser extent. We, therefore, screened the transcriptome profiles of 20 osteogenically induced adult human adipose-derived stem cell (ADSC) populations and investigated for putative transcription factors that could regulate the osteogenic differentiation of these ADSC. We studied a subgroup of donors' samples that had a disparate osteogenic response transcriptome from that of induced human fetal osteoblasts and the rest of the induced human ADSC samples. From our statistical analysis, we found activating transcription factor 5 (ATF5) to be significantly and consistently down-regulated in a randomized time-course study of osteogenically differentiated adipose-derived stem cells from human donor samples. Knockdown of ATF5 with siRNA showed an increased sensitivity to osteogenic induction. This evidence suggests a role for ATF5 in the regulation of osteogenic differentiation in adipose-derived stem cells. To our knowledge, this is the first report that indicates a novel role of transcription factors in regulating osteogenic differentiation in adult or tissue specific stem cells. © 2012 Wiley Periodicals, Inc.
Source Title: Journal of Cellular Biochemistry
ISSN: 07302312
DOI: 10.1002/jcb.24150
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