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|Title:||Androgenic and estrogenic response of green mussel extracts from Singapore's coastal environment using a human cell-based bioassay|
Persistent organic pollutants
Reporter gene bioassay
|Source:||Bayen, S., Gong, Y., Chin, H.S., Lee, H.K., Leong, Y.E., Obbard, J.P. (2004-11). Androgenic and estrogenic response of green mussel extracts from Singapore's coastal environment using a human cell-based bioassay. Environmental Health Perspectives 112 (15) : 1467-1471. ScholarBank@NUS Repository. https://doi.org/10.1289/ehp.6990|
|Abstract:||In the last decade, evidence of endocrine disruption in biota exposed to environmental pollutants has raised serious concern. Human cell-based bioassays have been developed to evaluate induced androgenic and estrogenic activities of chemical compounds. However, bioassays have been sparsely applied to environmental samples. In this study we present data on sex hormone activities in the green mussel, Perna viridis, in Singapore's coastal waters. P. viridis is a common bioindicator of marine contamination, and this study is a follow-up to an earlier investigation that reported the presence of sex hormone activities in seawater samples from Singapore's coastal environment. Specimens were collected from eight locations around the Singapore coastline and analyzed for persistent organic pollutants (POPS) and heavy metals. Tissue extracts were then screened for activities on androgen receptors (ARs) and estrogen receptors (ER-α and ER-β) using a reporter gene bioassay based on a HeLa human cell line. Mussel extracts alone did not exhibit AR activity, but in the presence of the reference androgenic hormone dihydrotestosterone (DHT), activities were up to 340% higher than those observed for DHT alone. Peak activities were observed in locations adjacent to industrial and shipping activities. Estrogenic activities of the mussel extract both alone and in the presence of reference hormone were positive. Correlations were statistically investigated between sex hormone activities, levels of pollutants in the mussel tissues, and various biological parameters (specimen size, sex ratio, lipid and moisture content). Significant correlations exist between AR activities, in the presence of DHT, and total concentration of POPS (r = 0.725, p < 0.05).|
|Source Title:||Environmental Health Perspectives|
|Appears in Collections:||Staff Publications|
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