Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/51596
Title: Fabrication of mesenchymal stem cell sheets for tendon/ligament tissue engineering
Authors: Ouyang, H.W. 
Toh, S.L. 
Goh, J.C.H. 
Tay, T.E. 
Nhan, P.T.
Issue Date: 2004
Source: Ouyang, H.W.,Toh, S.L.,Goh, J.C.H.,Tay, T.E.,Nhan, P.T. (2004). Fabrication of mesenchymal stem cell sheets for tendon/ligament tissue engineering. Transactions - 7th World Biomaterials Congress : 894-. ScholarBank@NUS Repository.
Abstract: The fabrication of mesenchymal stem cell sheets for tendon/ligament tissue engineering was analyzed. The cells were derived from bone marrow aspirated from the iliac crest. Nucleated cells were isolated by density gradient centrifugation over Ficoll/Paque. After mesenchymal stem cells were isolated from bone marrow by adherence to plastic, they were cultured in the presence of ascorbic acid. The cultured sheet of living cells was assembled with knitted scaffolds by wrapping technique and kept in cell culture condition for 4 weeks. The macro morphology, histology and biomechanics of the graft were evaluated. It was observed that the bone marrow derived adherent cells can form cell sheet quickly with the addition of ascorbic acid.
Source Title: Transactions - 7th World Biomaterials Congress
URI: http://scholarbank.nus.edu.sg/handle/10635/51596
ISBN: 1877040193
Appears in Collections:Staff Publications

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