Please use this identifier to cite or link to this item: https://doi.org/10.1007/7651-2012-1
Title: Propagation and differentiation of human Wharton's Jelly stem cells on three-dimensional nanofibrous scaffolds
Authors: Gauthaman, K.
Fong, C.-Y.
Venugopal, J.R. 
Biswas, A.
Ramakrishna, S. 
Bongso, A.
Keywords: Chondrocytes
Human Wharton's jelly stem cells
Immunohistochemistry
Mineralization
Nanofibrous scaffolds
Osteocytes
Real-time polymerase chain reaction
Issue Date: 2013
Source: Gauthaman, K.,Fong, C.-Y.,Venugopal, J.R.,Biswas, A.,Ramakrishna, S.,Bongso, A. (2013). Propagation and differentiation of human Wharton's Jelly stem cells on three-dimensional nanofibrous scaffolds. Methods in Molecular Biology 1058 : 1-23. ScholarBank@NUS Repository. https://doi.org/10.1007/7651-2012-1
Abstract: Stem cells isolated from the Wharton's jelly of the human umbilical cord (hWJSCs) are unique compared to other stem cell types as they lie in between embryonic stem cells (ESCs) and adult mesenchymal stem cells (MSCs) on the developmental map and share stemness markers of ESCs and MSCs. Yet, they do not induce tumorigenesis and are hypoimmunogenic and proliferative and fresh cell numbers can be harvested painlessly in abundance from discarded umbilical cords. Additionally, they secrete important soluble bioactive molecules from the interleukin and cell adhesion family, hyaluronic acid, collagen, glycosoaminoglycans, and chondroitin sulfate. Many of these molecules are involved in bone, cartilage, and joint repair. It has also been shown that hWJSCs attach, proliferate, and differentiate efficiently in the stem cell niches of three-dimensional matrices, particularly nanofibrous scaffolds. Thus, tissue constructs made up of hWJSCs and biodegradable nanofibrous scaffolds will facilitate clinical translation and improved functional outcome for arthritis, bone, and cartilage diseases. When applied in vivo, the hWJSCs from the tissue construct may improve repair either by differentiating into new chondrocytes or osteocytes and/or release of important factors that favor repair through paracrine functions. The nanofibrous scaffold is expected to provide the architecture and niches for the hWJSCs to perform and will later biodegrade encouraging engraftment. This chapter provides a step-by-step protocol for the preparation of such tissue constructs involving hWJSCs and nanofibrous scaffolds. The methodology also includes the targeted in vitro differentiation of the hWJSCs to osteogenic and chondrogenic lineages when attached to the nanofibrous scaffolds. © Springer Science+Business Media New York 2013.
Source Title: Methods in Molecular Biology
URI: http://scholarbank.nus.edu.sg/handle/10635/51507
ISBN: 9781627035705
ISSN: 10643745
DOI: 10.1007/7651-2012-1
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

4
checked on Dec 12, 2017

Page view(s)

44
checked on Dec 15, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.