Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/50363
Title: THE SPATIOTEMPORAL STUDY OF ZEBRAFISH INTESTINAL EPITHELIUM RENEWAL
Authors: SAHAR TAVAKOLI
Keywords: Zebrafish, Intestinal epithelium, Renewal, Mosaic generation
Issue Date: 30-Sep-2013
Citation: SAHAR TAVAKOLI (2013-09-30). THE SPATIOTEMPORAL STUDY OF ZEBRAFISH INTESTINAL EPITHELIUM RENEWAL. ScholarBank@NUS Repository.
Abstract: In contrast to the stereotypic villus-crypt organization of the bird and mammalian intestine, the zebrafish intestinal epithelium is folded into villar ridges and crypts are absent. This simple organization suggests that, similar to bird and mammalian, intestinal stem cells (ISCs) maintain the villus epithelium along a base-to-tip axis. Because there is no specific marker for zebrafish ISCs yet, we tested whether cells migrate from the base to the tip of the villi ridges by generating mosaic intestine tissues by EMS mutagen, chimera generation, EdU label retention and zebrabow transgenic line generation. The vertical stripes of the newly reproduced cells start their travel toward both flanking intestinal villi by completing their migration to the intestinal villus tip by 48 hours. As the sides of the adjacent intestinal villi flanking an intervillus pocket share the ISCs at the intervillus bottom, the adjacent intestinal villi show the similar expression pattern.
URI: http://scholarbank.nus.edu.sg/handle/10635/50363
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
supplementary video 1.mp46.82 MBMP4

OPEN

None Preview online
View/Download
supplementary video 2.mp42.79 MBMP4

OPEN

None Preview online
View/Download
TavakoliS.pdf9.35 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

181
checked on Sep 7, 2018

Download(s)

102
checked on Sep 7, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.