Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/49645
Title: UNDERSTANDING ACTIN FUNCTION DURING CYTOKINESIS IN FISSION YEAST
Authors: HUANG JUNQI
Keywords: actin, cytokinesis, actomyosin ring assembly, actin cable, myosins, fission yeast
Issue Date: 22-Aug-2013
Source: HUANG JUNQI (2013-08-22). UNDERSTANDING ACTIN FUNCTION DURING CYTOKINESIS IN FISSION YEAST. ScholarBank@NUS Repository.
Abstract: Cytokinesis is the last step for cell division. Actomyosin ring, the physical apparatus to separate the two daughter cells during cytokinesis, is studied for decades. Fission yeast actomyosin ring contains many proteins, among which actin is considered one of the most basic components. Previous attempts to tag actin failed due to its intolerance of any ectopic manipulation. In this thesis, a new technology called lifeact was successfully applied in fission yeast to study actin dynamics. Surprisingly, by time-lapse confocal microscopy, it is found that non-medial actin cables migrated toward the medial region and joint ring formation during cytokinesis. This result contradicted the two existing fission yeast ring assembly models, in which actin cables were considered dispensable. Further experiments in this thesis focused on the spatial and temporal relationship between the non-medial cables and medial nodes, protein responsible for the nucleation of these cables and myosins involved in cable organization etc.
URI: http://scholarbank.nus.edu.sg/handle/10635/49645
Appears in Collections:Ph.D Theses (Open)

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