Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/49633
Title: CHARACTERIZATION OF FACTORS INVOLVED IN HOST-VIRAL INTERACTIONS AND HOW THEY CONTRIBUTE TO HEPATITIS C VIRUS-INDUCED CELL DEATH
Authors: AWEYA JUDE JUVENTUS
Keywords: Hepatitis C virus, p7 protein, apoptosis, BIK
Issue Date: 28-Nov-2013
Source: AWEYA JUDE JUVENTUS (2013-11-28). CHARACTERIZATION OF FACTORS INVOLVED IN HOST-VIRAL INTERACTIONS AND HOW THEY CONTRIBUTE TO HEPATITIS C VIRUS-INDUCED CELL DEATH. ScholarBank@NUS Repository.
Abstract: Chronic hepatitis C virus (HCV) infection causes liver injury and diseases, with the liver injury believed to be linked with hepatocytes apoptosis. However, the mechanisms behind this process are currently unclear, although both host and viral factors have been implicated. In this thesis, we sought to delineate which viral and host factors perpetuate and regulate the cell death process. Here we report that, HCV p7 induces caspase-dependent apoptosis independent of its ion channel activity. P7 could not however activate autophagic cell death like the M2 protein of influenza A virus, although they share some similarities. Nine host apoptosis-related genes were identified to be important in HCV infection. Subsequent analysis of these genes demonstrated a concomitant mRNA and protein expression for 6 genes, with concordance for only BIK. However, BIK depletion did not protect Huh7.5 cells against HCV-induced apoptosis, although, it reduced viral replication, but had minimal effect on viral release.
URI: http://scholarbank.nus.edu.sg/handle/10635/49633
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