Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/49542
Title: CHARACTERIZATION OF HURP FUNCTION IN ZEBRAFISH GASTRULATION
Authors: LORA TAN
Keywords: Hurp, zebrafish, gastrulation, C&E, epiboly, microtubules
Issue Date: 15-Aug-2013
Source: LORA TAN (2013-08-15). CHARACTERIZATION OF HURP FUNCTION IN ZEBRAFISH GASTRULATION. ScholarBank@NUS Repository.
Abstract: The role of Hurp during embryonic development is studied in zebrafish. Reduction of zebrafish Hurp protein levels using translational blocking morpholino (MO) results in defective convergence and extension (C&E) movements during gastrulation without affecting cell proliferation and cell fates. Hurp is required for several polarized cell behaviours during C&E including fast dorsal directed migration of lateral mesodermal and ectodermal cells and mediolateral intercalation of dorsolateral mesodermal cells in late zebrafish gastrulae. In addition, Hurp regulates polarized cell division orientation in the paraxial epiblast region of late zebrafish gastrulae. Besides, Hurp mediates epiboly progression by regulating extraembryonic cytoskeletal structure organization including yolk cell microtubules and yolk syncytial layer F-actin. My findings provide new insights into the role of Hurp in regulating polarized cell movement, polarized cell division, and extraembryonic cytoskeletal structure organization for morphogenetic cell movements during zebrafish gastrulation.
URI: http://scholarbank.nus.edu.sg/handle/10635/49542
Appears in Collections:Ph.D Theses (Open)

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