Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/49367
Title: STRUCTURAL MECHANISMS OF DOUBLE-STRANDED RNA PROCESSING AND LOADING WITHIN RNA INTERFERENCE PATHWAY
Authors: FU QINQIN
Keywords: Structural biology, double-stranded RNA, RNA processing, small RNA loading, RISC assembly, RNA interference
Issue Date: 22-Aug-2013
Source: FU QINQIN (2013-08-22). STRUCTURAL MECHANISMS OF DOUBLE-STRANDED RNA PROCESSING AND LOADING WITHIN RNA INTERFERENCE PATHWAY. ScholarBank@NUS Repository.
Abstract: RNA interference (RNAi) is a natural biological process that cells use small RNA molecules to regulate gene expression. In this thesis, we present the structural and functional studies on two proteins involved in RNAi, the RNA Helicase A (RHA or DHX9) from Homo sapiens and the budding yeast Dicer-like protein from P. stipitis (PsDCR1). RHA was reported to function as an RISC-loading factor in the RISC assembly step of RNAi and such function is mediated mainly by its double-stranded RNA binding domains (dsRBDs). Meanwhile, the PsDCR1is hypothesized to participate in the dicing step of RNAi in P. stipitis. Our structural and functional efforts on RHA reveal a potential working model for siRNA recognition by RHA tandem dsRBDs, and together they provide direct structural insights into RISC assembly facilitated by RHA. In addition, our structural and functional studies of PsDCR1 shed light on the function of PsDCR1.
URI: http://scholarbank.nus.edu.sg/handle/10635/49367
Appears in Collections:Ph.D Theses (Open)

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