Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/49144
Title: Development and Application of a High-Throughput Assay for Discovery of Starch Hydrolase Inhibitors
Authors: LIU TINGTING
Keywords: Diabetes, amylase, glucosidase, inhibitors, starch, turbidity
Issue Date: 29-Jul-2013
Source: LIU TINGTING (2013-07-29). Development and Application of a High-Throughput Assay for Discovery of Starch Hydrolase Inhibitors. ScholarBank@NUS Repository.
Abstract: For in vitro diabetes assay, to search for the active ingredients in low Glycemic Index foods, a 96-well based high-throughput method (HTS) for rapidly measuring the inhibition of starch hydrolase was developed, by monitoring the decrease in turbidity over time during the enzymatic digestion of starch. By applying HTS, extracts of wild buckwheat root exhibited strong starch hydrolase inhibitory activity, and diboside A was isolated as a selective inhibitor of pancreatic a-amylase and sucrase. Moreover, proanthocyanidins in the extracts of root were also found to be major contributors to the inhibition of starch hydrolase. Phenylpropanoid sucrose ester compounds, smilaside A and smilaside D were isolated from extracts of the Smilax glabra rhizome. Both compounds displayed negligible inhibitory effects towards porcine pancreas a-amylase, but weak and moderate inhibitory activity towards rat intestinal a-glucosidase. The use of grape seeds extracts as a-amylase inhibitors using wheat noodles as the food matrix was investigated.
URI: http://scholarbank.nus.edu.sg/handle/10635/49144
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
LiuT.pdf3 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

143
checked on Feb 23, 2018

Download(s)

457
checked on Feb 23, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.