Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/48883
Title: INVESTIGATIONS OF HELICASE ACTIVITIES IN PRIMORDIAL THERMOCYCLING SYSTEMS
Authors: LIAO XUANHAO
Keywords: Origin of life, RNA world, evolution of the translation, RNA helicase hypothesis, chronology of the ribosome, r29
Issue Date: 26-Jun-2013
Source: LIAO XUANHAO (2013-06-26). INVESTIGATIONS OF HELICASE ACTIVITIES IN PRIMORDIAL THERMOCYCLING SYSTEMS. ScholarBank@NUS Repository.
Abstract: In Zenkin?s RNA helicase hypothesis, the small subunit of the ribosome is proposed to have evolved from an ancient ribozyme, an RNA helicase, in the primordial RNA world, which most likely located inside primitive submarine hydrothermal vents. And this RNA helicase is also proposed to be conserved in some core portion of the modern ribosomal small subunit, as a counterpart to the PTC of the large subunit. The chronological study of the E. coli ribosome of our bioinformatics lab has revealed two core portions of the small subunit, r23 and r29, between which r29 is the potential candidate as the remnant of the primordial RNA helicase. Via in vitro transcription, r29 and some other related RNAs were artificially synthesized. And their potential helicase activities were tested with molecular beacons in thermocycling systems, as experimental demonstrations of Zenkin?s RNA helicase hypothesis.
URI: http://scholarbank.nus.edu.sg/handle/10635/48883
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