Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/48844
Title: STUDY OF LIPID METABOLISM IN CORYNEBACTERINEAE USING LIPIDOMICS AND PROTEOMICS APPROACH
Authors: SUDARKODI SUKUMAR
Keywords: Mycolic acids,Tuberculosis,Rhodococcus,Lipidomics,Mass spectrometry,Proteomics
Issue Date: 12-Apr-2013
Source: SUDARKODI SUKUMAR (2013-04-12). STUDY OF LIPID METABOLISM IN CORYNEBACTERINEAE USING LIPIDOMICS AND PROTEOMICS APPROACH. ScholarBank@NUS Repository.
Abstract: The cell envelope is multilayered, complex, and uniquely lipid rich in Corynebacterineae suborder, comprising major genera Mycobacterium and Rhodococcus. The major components of the cell envelope are mycolic acids (MA) along with a wide array of free lipids. The Corynebacterineae genome is devoted extensively to the metabolism of these complex lipids. Lipid manipulation is one of the strategies used by Mycobacterium tuberculosis (Mtb), the etiological agent of Tuberculosis to evade host immune attack. In this project, lipidomics approach was employed to study the MA adaptation of Mtb in macrophages following infection. R.opacus PD630 is a non-pathogenic member of the suborder, significant for its energy lipids production. Despite the industrial significance, the lipid and lipid metabolizing enzyme inventory of R.opacus PD630 is less explored. Lipidomics enabled the definition of MA, acyl glycerol and fatty acid fingerprint of R.opacus and seven other Rhodococcus species. Using active site directed proteomics, tetrahydrolipstatin analogues were used to identify 25 proteins from hydrolases, oxidoreductases and ligase enzyme families. Collectively, a combination of omics methodology facilitated to study different aspects of lipid metabolism in Corynebacterineae.
URI: http://scholarbank.nus.edu.sg/handle/10635/48844
Appears in Collections:Ph.D Theses (Open)

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