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Title: Generation of flavor esters from coconut lipids by lipase-mediated biocatalysis
Keywords: Flavor ester, lipase, biocatalysis, coconut oil, fusel oil, coconut cream
Issue Date: 1-Aug-2013
Source: SUN JINGCAN (2013-08-01). Generation of flavor esters from coconut lipids by lipase-mediated biocatalysis. ScholarBank@NUS Repository.
Abstract: Using lipases as the biocatalysts to synthesize flavor esters from low-cost coconut lipid were studied. The ester synthesis by lipases (Lipozyme TL IM and Palatase) was investigated in both solvent-free oil system (coconut oil and fusel oil) and aqueous system (coconut cream and fusel oil). Response surface methodology and the Taguchi method were used to determine the relationships between yield of esters and reaction parameters. To gain a better understanding of reaction mechanisms of lipase-catalyzed ester synthesis, the catalytic behaviors of lipases in different reaction media, including oil system, aqueous medium and buffer, were investigated. It was found that the catalytic behaviors of lipases were greatly affected by the substrate polarities. The obtained kinetic data indicated that in solvent-free oil system, Lipozyme TL IM-catalyzed transesterification was possibly a two-step reaction (hydrolysis and esterification). Useful information was provided in this study for the further industrial production of these flavor esters.
Appears in Collections:Ph.D Theses (Open)

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