Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/48377
Title: INVESTIGATION OF THE FUNCTIONS OF P23 AND COAT PROTEIN OF HIBISCUS CHLOROTIC RINGSPOT VIRUS
Authors: GAO RUIMIN
Keywords: HCRSV, p23, coat protein, viral miRNA, Sulfur enhanced defence, NLS
Issue Date: 5-Aug-2013
Source: GAO RUIMIN (2013-08-05). INVESTIGATION OF THE FUNCTIONS OF P23 AND COAT PROTEIN OF HIBISCUS CHLOROTIC RINGSPOT VIRUS. ScholarBank@NUS Repository.
Abstract: A novel open reading frame p23 in Hibiscus chlorotic ringspot virus (HCRSV) was found to localize in the nucleus and a novel nuclear localization signal (NLS) was discovered. Any of the basic aa mutations in the NLS region which abolished p23 nuclear localization also inhibit HCRSV long distance movement. We showed that the (+)-sense single-stranded RNA viruses HCRSV genome was also present in the nucleus which contradicts to the common notion. The NLS interacts with importin a and facilitates HCRSV RNA genome to enter nucleus where viral-miRNAs are produced. Furthermore, we established that HCRSV-CP interacts with sulfite oxidase (SO) which in turn triggers sulfur enhanced defence in the host Hibiscus cannabinus L.. The argonaute 1 (AGO1) gene was also upregulated upon SO overexpression which results from the interaction of HCRSV-CP and SO. AGO1 feedback regulates miRNAs and their respective target genes, which is correlated to growth retardation of HCRSV-infected plants.
URI: http://scholarbank.nus.edu.sg/handle/10635/48377
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