Please use this identifier to cite or link to this item:
|Title:||A 1.7 Å structure of Fve, a member of the new fungal immunomodulatory protein family|
|Keywords:||3-D domain swapping|
Fungal immunomodulatory protein
|Citation:||Paaventhan, P., Joseph, J.S., Kolatkar, P.R., Seow, S.V., Chua, K.Y., Vaday, S., Robinson, H. (2003). A 1.7 Å structure of Fve, a member of the new fungal immunomodulatory protein family. Journal of Molecular Biology 332 (2) : 461-470. ScholarBank@NUS Repository. https://doi.org/10.1016/S0022-2836(03)00923-9|
|Abstract:||Fve, a major fruiting body protein from Flammulina velutipes, a mushroom possessing immunomodulatory activity, stimulates lymphocyte mitogenesis, suppresses systemic anaphylaxis reactions and edema, enhances transcription of IL-2, IFN-γ and TNF-α, and hemagglutinates red blood cells. It appears to be a lectin with specificity for complex cell-surface carbohydrates. Fve is a non-covalently linked homodimer containing no Cys, His or Met residues. It shares sequence similarity only to the other fungal immunomodulatory proteins (FIPs) LZ-8, Gts, Vvo and Vvl, all of unknown structure. The 1. 7Å structure of Fve solved by single anomalous diffraction of NaBr-soaked crystals is novel: each monomer consists of an N-terminal α-helix followed by a fibronectin III (FNIII) fold. The FNIII fold is the first instance of "pseudo-h-type" topology, a transition between the seven β-stranded s-type and the eight β-stranded h-type topologies. The structure suggests that dimerization, critical for the activity of FIPs, occurs by 3-D domain swapping of the N-terminal helices and is stabilized predominantly by hydrophobic interactions. The structure of Fve is the first in this lectin family to be reported, and the first of an FNIII domain-containing protein of fungal origin. © 2003 Elsevier Ltd. All rights reserved.|
|Source Title:||Journal of Molecular Biology|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on May 21, 2018
WEB OF SCIENCETM
checked on May 7, 2018
checked on May 25, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.