Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0264-410X(02)00485-1
Title: Expression and immunogenicity of the major house dust mite allergen Der p 1 following DNA immunization
Authors: Wolfowicz, C.B. 
HuangFu, T.
Chua, K.Y. 
Keywords: Der p 1
DNA vaccine
Naked DNA
Issue Date: 2003
Source: Wolfowicz, C.B., HuangFu, T., Chua, K.Y. (2003). Expression and immunogenicity of the major house dust mite allergen Der p 1 following DNA immunization. Vaccine 21 (11-12) : 1195-1204. ScholarBank@NUS Repository. https://doi.org/10.1016/S0264-410X(02)00485-1
Abstract: The mite protein Der p 1 is a major trigger of allergy and atopic asthma world-wide, and thus, a good vaccine candidate for allergy prevention. Since it is a cysteine protease, the catalytic effects of Der p 1 vaccination may be unpredictable. One approach to reduce this risk is to vaccinate with DNA encoding enzymatically inactive forms of Der p 1. Here we show that Der p 1 DNA without its native pre-pro sequences potently induced Der p 1-specific antibodies, as long as its pre-sequence was substituted by another leader sequence. Without any pre-pro sequence, the same DNA fragment was well expressed but failed to induce significant level of anti-Der p 1 antibodies, without further boosting by protein. © 2002 Elsevier Science Ltd. All rights reserved.
Source Title: Vaccine
URI: http://scholarbank.nus.edu.sg/handle/10635/47896
ISSN: 0264410X
DOI: 10.1016/S0264-410X(02)00485-1
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