Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0264-410X(02)00485-1
Title: Expression and immunogenicity of the major house dust mite allergen Der p 1 following DNA immunization
Authors: Wolfowicz, C.B. 
HuangFu, T.
Chua, K.Y. 
Keywords: Der p 1
DNA vaccine
Naked DNA
Issue Date: 2003
Citation: Wolfowicz, C.B., HuangFu, T., Chua, K.Y. (2003). Expression and immunogenicity of the major house dust mite allergen Der p 1 following DNA immunization. Vaccine 21 (11-12) : 1195-1204. ScholarBank@NUS Repository. https://doi.org/10.1016/S0264-410X(02)00485-1
Abstract: The mite protein Der p 1 is a major trigger of allergy and atopic asthma world-wide, and thus, a good vaccine candidate for allergy prevention. Since it is a cysteine protease, the catalytic effects of Der p 1 vaccination may be unpredictable. One approach to reduce this risk is to vaccinate with DNA encoding enzymatically inactive forms of Der p 1. Here we show that Der p 1 DNA without its native pre-pro sequences potently induced Der p 1-specific antibodies, as long as its pre-sequence was substituted by another leader sequence. Without any pre-pro sequence, the same DNA fragment was well expressed but failed to induce significant level of anti-Der p 1 antibodies, without further boosting by protein. © 2002 Elsevier Science Ltd. All rights reserved.
Source Title: Vaccine
URI: http://scholarbank.nus.edu.sg/handle/10635/47896
ISSN: 0264410X
DOI: 10.1016/S0264-410X(02)00485-1
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

24
checked on Oct 16, 2018

WEB OF SCIENCETM
Citations

22
checked on Oct 8, 2018

Page view(s)

66
checked on Sep 29, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.