Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/47725
Title: BIOLOGY, ISOLATION, CULTURE AND REPROGRAMMING EFFICIENCY OF HUMAN FETAL PRIMITIVE ERYTHROBLASTS FOR NON-INVASIVE PRENATAL DIAGNOSIS
Authors: HUANG ZHOUWEI
Keywords: Primitive erythroblast, prenatal diagnosis, biology, isolation, reprogramming, non-invasive
Issue Date: 25-Jan-2013
Source: HUANG ZHOUWEI (2013-01-25). BIOLOGY, ISOLATION, CULTURE AND REPROGRAMMING EFFICIENCY OF HUMAN FETAL PRIMITIVE ERYTHROBLASTS FOR NON-INVASIVE PRENATAL DIAGNOSIS. ScholarBank@NUS Repository.
Abstract: Non-invasive prenatal diagnosis (NIPD) has been pursued for more than two decades to overcome the risk of miscarriage associated with current invasive prenatal diagnosis methods. Human fetal primitive erythroblast (hEryP) present in the maternal blood is considered as an ideal target for first trimester NIPD due to its distinct features for identification. However, the lack of knowledge in this cell type and their small numbers in the maternal circulation hamper the progress of their clinical use. My research work aims to obtain a better understanding of hEryPs and provide solutions for their use in NIPD. It covers (1) an in-depth study of the biological properties of hEryPs; (2) development of a manual isolation method of pure hEryPs from the maternal blood and a novel microdroplet culture system for in vitro maintenance; (3) evaluation of nuclear reprogramming efficiency of hEryPs with viral and non-viral approaches.
URI: http://scholarbank.nus.edu.sg/handle/10635/47725
Appears in Collections:Ph.D Theses (Open)

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