Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/47645
Title: KERATIN REMODELING IN STRESS
Authors: TAN TONG SAN
Keywords: Keratin remodeling, epidermolysis bullosa simplex, wound response, EGF, keratin phosphorylation, cell migration
Issue Date: 13-Aug-2012
Source: TAN TONG SAN (2012-08-13). KERATIN REMODELING IN STRESS. ScholarBank@NUS Repository.
Abstract: Remodeling of keratin cytoskeletal network is essential for cells to respond to environmental cues. To understand the stress/wound response, immortalized keratinocytes are used which expressed a keratin mutation mimicking severe EBS Dowling-Meara (a skin fragility disorder), fluorescently tagged for live-cell imaging. All keratinocytes are ¿activated¿ to a stress state through up-regulation of kinases and wound response proteins, but revert to quiescence after confluence. In mutant cells, ¿activation¿ is reflected by the presence of keratin aggregates, a hallmark of severe EBS-DM. It is thought that avoiding aggregate formation may improve the disease phenotype. It is observed that EGF influences keratin aggregate formation/dynamics in mutant keratinocytes, through changes in plectin expression, during cell migration. Phosphorylation at K14 Y129 is found to play a role in keratin aggregate formation, modulating cell migration during scratch wound assays. This thesis demonstrates how plasticity of the keratin cytoskeleton can modulate the skin cell¿s response to environmental cues.
URI: http://scholarbank.nus.edu.sg/handle/10635/47645
Appears in Collections:Ph.D Theses (Open)

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