Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/47637
Title: APPLICATION OF TOPOGRAPHY TO DIRECT DIFFERENTIATION OF PLURIPOTENT STEM CELLS
Authors: CHAN LESLEY YIN-TING
Keywords: Flow Cytometry, Human Embryonic Stem Cells, Surface Topography, Neural Differentiation, Micropatterning, Nanotopography
Issue Date: 3-Aug-2012
Source: CHAN LESLEY YIN-TING (2012-08-03). APPLICATION OF TOPOGRAPHY TO DIRECT DIFFERENTIATION OF PLURIPOTENT STEM CELLS. ScholarBank@NUS Repository.
Abstract: Human pluripotent stem cells (hPSCs) have great potential for neurological repair and regeneration, as they can be generated in large quantities then differentiated into any neural subtype. In this study, we have explored the use of topographical cues to improve current neural differentiation protocols and developed two flow cytometry analysis strategies ¿ the normalized median fluorescence intensity and fixed fluorescence intensity gating strategies ¿ to more accurately and precisely track the changes in pluripotent and lineage-specific marker expression during hPSC differentiation. Using these two methodologies, we have determined that the topographical cues from 2µm gratings increase the rate of hPSC neural differentiation, and that additional periods of culture on topography can further increase the rate of hPSC neural differentiation. We envisage that this work can be applied to future neural differentiation protocols to further increase the yield and efficiency, as well as decrease the differentiation period and the quantity of biochemical cues.
URI: http://scholarbank.nus.edu.sg/handle/10635/47637
Appears in Collections:Ph.D Theses (Open)

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