Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/47634
Title: DIRECTED REPROGRAMMING OF FIBROBLASTS INTO HEMATOPOIETIC PROGENITORS BY NUCLEAR REGULATORS
Authors: ANG HEATHER YIN-KUAN
Keywords: Reprogramming, Hematopoiesis
Issue Date: 17-Dec-2012
Source: ANG HEATHER YIN-KUAN (2012-12-17). DIRECTED REPROGRAMMING OF FIBROBLASTS INTO HEMATOPOIETIC PROGENITORS BY NUCLEAR REGULATORS. ScholarBank@NUS Repository.
Abstract: The etiologies of inordinate diseases lay within the deficiency of a specific cell type, and accordingly such disorders should be stably treatable by direct replacement of the missing cell type. Creation of self-renewing progenitors capable of continually producing the deficient cell type of interest will enable treatment of such diseases. Here, we have directly reprogrammed mouse fibroblast into multipotent hematopoietic progenitors by the overexpression of six hematopoietic nuclear regulators (Bmi1, Gata2, HoxB4, Gfi1, etc). These reprogrammed hematopoietic progenitors display diagnostic hematopoietic stem cell surface antigens and are capable of differentiating into the erythroid and myeloid lineages in vitro. Importantly, reprogrammed progenitors are capable of engraftment and hematopoietic contribution after transplantation into recipient mice, thus designating them as authentic hematopoietic progenitors. The capability to create multipotent hematopoietic progenitors through in vitro reprogramming will enable novel opportunities for autologous cell replacement therapies such as bone marrow transplantations.
URI: http://scholarbank.nus.edu.sg/handle/10635/47634
Appears in Collections:Ph.D Theses (Open)

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