Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/47633
Title: DELINEATION OF THE ROLE OF ABCG2 TRANSPORTER IN IDENTIFICATION OF HUMAN EPIDERMAL STEM CELLS
Authors: MA DONGRUI
Keywords: human, epidermis, keratinocyte, stem cell, cell culture, ABCG2
Issue Date: 14-May-2012
Source: MA DONGRUI (2012-05-14). DELINEATION OF THE ROLE OF ABCG2 TRANSPORTER IN IDENTIFICATION OF HUMAN EPIDERMAL STEM CELLS. ScholarBank@NUS Repository.
Abstract: This thesis describes a series of experiments that were designed to improve the generation and handling of skin stem cells used for clinical transplantation. In the first part of this thesis, a novel feeder-free and serum-free culture system for keratinocytes was developed, which excluded animal products and simplify the current keratinocytes cultivation protocol. Next, in an attempt to purify epidermal stem cells from human hair follicle, a population of hair follicle sheath derived mesenchymal stem cells was identified and characterized. The major part of this thesis is searching for specific cell surface markers which could specifically identify and enrich human epidermal stem cells. Focusing on human interfollicular epidermis, the potential of using ABCG2 to purify epidermal stem cells was investigated. The improved cultivation and enrichment of epidermal stem cells described here will benefit patients and pave the way for a safer method of cultivating epidermal stem cells for clinical applications.
URI: http://scholarbank.nus.edu.sg/handle/10635/47633
Appears in Collections:Ph.D Theses (Open)

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