Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/47630
Title: A TISSUE ENGINEERING APPROACH TO THE DEVELOPMENT OF AN IN VITRO 3D TUMOR MODEL FOR DRUG DELIVERY STUDIES
Authors: TAN HWEE SIM PAMELA
Keywords: three-dimensional tumor model, cytotoxicity, angiogenesis, cell cycle arrest, bioreactor, stromal cells
Issue Date: 23-Aug-2012
Source: TAN HWEE SIM PAMELA (2012-08-23). A TISSUE ENGINEERING APPROACH TO THE DEVELOPMENT OF AN IN VITRO 3D TUMOR MODEL FOR DRUG DELIVERY STUDIES. ScholarBank@NUS Repository.
Abstract: The lack of good preclinical models has hampered anticancer drug discovery. Standard preclinical protocols require the growth of cells in high throughput two-dimensional culture systems. Such in vitro drug testing methods yield drug efficacy results that differ greatly from animal models. It is conceivable that three-dimensional growth may be responsible for some of these changes and that such growth may form a reproducible and more representative step in tumoricidal validation prior to animal implantation. The role of three-dimensional culture on angiogenic potential and tumor growth was studied and high throughput anticancer or anti-angiogenic drug screening was also carried out. A co-culture model with immortalized fibroblasts and endothelial cells was used to study the effects of the supporting stroma on cancer cell behavior. The tumor constructs were cultured in a bioreactor with replicated interstitial fluid pressure, and these constructs were found to closely approximate the subcutaneous tumors in SCID mice.
URI: http://scholarbank.nus.edu.sg/handle/10635/47630
Appears in Collections:Ph.D Theses (Open)

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