Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/47551
Title: MICROFLUIDIC QUANTIFICATION OF CELL SURFACE RECEPTORS
Authors: RAMESH RAMJI
Keywords: Microfluidics, cell surface receptor, cell based assay, receptor quantification, ligand receptor binding, bioluminescence
Issue Date: 24-Jan-2013
Source: RAMESH RAMJI (2013-01-24). MICROFLUIDIC QUANTIFICATION OF CELL SURFACE RECEPTORS. ScholarBank@NUS Repository.
Abstract: The interaction of cell surface receptors with extracellular ligands or biomolecules potentially triggers a cascade of signaling pathways leading to varied functional responses in both healthy and diseased cell states. Gradients of biomolecular availability targeting these receptors alter signaling responses among cells. Current cell based microfluidic assays which mimic in-vivo biomolecular gradients, lack an understanding of how the binding and transport between ligand and receptor occurs. Methods to quantify the surface receptor numbers and biomolecular dissociation rates on live cells have not been explored yet. This thesis addresses the above issues by presenting three novel microfluidic cell based assays with theoretical and experimental insights: the bolus induced gradient generator establishing long range biomolecular gradients for cell signaling studies; the microfluidic displacement assay for live cell quantification of kinetic dissociation and internalization rates; the microfluidic bioluminescent assay integrated with a lens-free optical system for on-chip quantification of live cell surface receptors.
URI: http://scholarbank.nus.edu.sg/handle/10635/47551
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