Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/47520
Title: A MICRORNA-BASED ANTIVIRAL AGAINST WEST NILE VIRUS
Authors: YEO KIM LONG
Keywords: West Nile virus, dsRNA, IRF-2, HLA-E, microRNA, antiviral
Issue Date: 3-Jul-2012
Source: YEO KIM LONG (2012-07-03). A MICRORNA-BASED ANTIVIRAL AGAINST WEST NILE VIRUS. ScholarBank@NUS Repository.
Abstract: West Nile virus (WNV) is a mosquito-transmitted virus that causes severe neuroinvasive diseases like meningitis and potentially fatal encephalitis. Despite intensified research efforts over the years, there is still no vaccine or antiviral for the prevention and treatment of debilitating diseases caused by this virus. This study shows that large amount of double-stranded RNA intermediates present during WNV replication up-regulates interferon regulatory factor-2. As a result, the inhibitory HLA-E molecule is up-regulated in a IRF-2-TAP-1 dependent manner. This can create a favourable environment for virus replication. The possibility of a miRNA-adapted shRNA-based approach to inhibit virus replication by reducing the amount of viral RNA was then investigated. Results showed that molecules designed against the whole viral genome, highly conserved regions of the viral genome, or the negative-stranded viral RNA significantly reduced the amount of virus formed. Lastly, a novel method of vector modification enhanced the efficacy of these molecules.
URI: http://scholarbank.nus.edu.sg/handle/10635/47520
Appears in Collections:Ph.D Theses (Open)

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00 Cover and Contents.pdf673.81 kBAdobe PDF

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01 Literature Review.pdf3.92 MBAdobe PDF

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02 Materials and Methods.pdf2.26 MBAdobe PDF

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03 IRF-2 can aid WNV progression.pdf10.4 MBAdobe PDF

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04 Pilot design and test of miRNA-adapted shRNA technology to target WNV.pdf6.99 MBAdobe PDF

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05 Design and test of miRNA-adapted shRNA technology to target consensus regions of WNV.pdf11.62 MBAdobe PDF

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06 Discussion.pdf460.83 kBAdobe PDF

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07 References.pdf244.11 kBAdobe PDF

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08 Appendices.pdf4.35 MBAdobe PDF

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