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Title: Cytotoxicity of silver nanoparticles in human embryonic stem cell-derived fibroblasts and an L-929 cell line
Authors: Peng, H.
Zhang, X.
Wei, Y.
Liu, W.
Li, S.
Yu, G.
Fu, X.
Cao, T. 
Deng, X.
Issue Date: 2012
Citation: Peng, H., Zhang, X., Wei, Y., Liu, W., Li, S., Yu, G., Fu, X., Cao, T., Deng, X. (2012). Cytotoxicity of silver nanoparticles in human embryonic stem cell-derived fibroblasts and an L-929 cell line. Journal of Nanomaterials 2012 : -. ScholarBank@NUS Repository.
Abstract: Consensus about the toxicity of silver nanoparticles (Ag-NPs) has not been reached, even though extensive attention has been paid to this issue. This confusion may be due to physicochemical factors of Ag-NPs and the cell model used for biological safety evaluation. In the present study, human embryonic stem cell-derived fibroblasts (EBFs), which have been considered a closer representative of the in vivo response, were used as a novel cell model to assess the cytotoxicity of Ag-NPs (∼ 20nm and 100nm) in comparison with L-929 fibroblast cell line. Cell proliferation, cell cycle, apoptosis, p53 expression, and cellular uptake were examined. Results showed that Ag-NPs presented higher cytotoxicity to EBF than to L-929. EBF demonstrated a stronger capacity to ingest Ag-NPs, a higher G2/M arrest, and more upgraduated p53 expression after exposed to Ag-NPs for 48 h when compared with L-929. It could be concluded that EBF exhibited a more sensitive response to Ag-NPs compared with L-929 cells, indicating that EBF may be a valid candidate for cytotoxicity screening assays of nanoparticles. Copyright © 2012 Hui Peng et al.
Source Title: Journal of Nanomaterials
ISSN: 16874110
DOI: 10.1155/2012/160145
Appears in Collections:Staff Publications

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