Please use this identifier to cite or link to this item: https://doi.org/10.3109/03008207.2012.751985
Title: Controlling the proliferation and differentiation stages to initiate periodontal regeneration
Authors: Chong, L.Y.
Chien, L.-Y.
Chung, M.-C.
Liang, K.
Lim, J.C.-S.
Fu, J.H.
Wang, C.-H.
Chang, P.-C. 
Keywords: Cell proliferation
Drug delivery
Periodontal regeneration
Platelet-derived growth factor
Simvastatin
Issue Date: 2013
Source: Chong, L.Y., Chien, L.-Y., Chung, M.-C., Liang, K., Lim, J.C.-S., Fu, J.H., Wang, C.-H., Chang, P.-C. (2013). Controlling the proliferation and differentiation stages to initiate periodontal regeneration. Connective Tissue Research 54 (2) : 101-107. ScholarBank@NUS Repository. https://doi.org/10.3109/03008207.2012.751985
Abstract: The success of periodontal regeneration depends on the coordination of early cell proliferation and late cell differentiation. The aim of this study was to investigate whether the proliferation or differentiation stage predominantly promotes the initiation of periodontal regeneration. Critical-sized periodontal defects were surgically created on rat maxillae and filled with poly-(d,l-lactide-co-glycolide)-poly-d,l-lactide hybrid microspheres encapsulating platelet-derived growth factor (PDGF, a promoter of mitogenesis), simvastatin (a promoter of osteogenic differentiation), or bovine serum albumin (a control). The encapsulation efficiency and in vitro release profiles of the microspheres were determined by high-performance liquid chromatography and enzyme-linked immunosorbent assay. The maxillae were harvested after 10 or 14 days and assessed by micro-computed tomography, histology, and immunohistochemistry for regeneration efficacy and cell viability. The rapid release of PDGF was observed within the first week, whereas a slow release profile was noted for simvastatin. The PDGF-treated specimens demonstrated a significantly higher bone volume fraction compared with bovine serum albumin- (p < 0.05) or simvastatin-treated (p < 0.05) specimens at day 14. Histologically, active bone formation originating from the defect borders was noted in both the PDGF- and the simvastatin-treated specimens, and functionally aligned periodontal ligament fiber insertion was only observed in the PDGF-treated specimens. The significant promotion of mitogenesis by PDGF treatment was also noted at day 14 (p < 0.05). In conclusion, increased mitogenesis or osteogenic differentiation may stimulate osteogenesis, and the upregulation of mitogenesis by PDGF appears to play a role in the initiation of periodontal regeneration. © 2013 Informa Healthcare USA, Inc.
Source Title: Connective Tissue Research
URI: http://scholarbank.nus.edu.sg/handle/10635/47156
ISSN: 03008207
DOI: 10.3109/03008207.2012.751985
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

6
checked on Dec 6, 2017

WEB OF SCIENCETM
Citations

2
checked on Nov 21, 2017

Page view(s)

88
checked on Dec 10, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.