Please use this identifier to cite or link to this item: https://doi.org/10.1177/0022034512459053
Title: Characterization of riboflavin-modified dentin collagen matrix
Authors: Fawzy, A. 
Nitisusanta, L.
Iqbal, K.
Daood, U.
Beng, L.T.
Neo, J. 
Keywords: AFM
biodegradation
crosslinking
dentin adhesives
riboflavin
ultraviolet
Issue Date: 2012
Source: Fawzy, A., Nitisusanta, L., Iqbal, K., Daood, U., Beng, L.T., Neo, J. (2012). Characterization of riboflavin-modified dentin collagen matrix. Journal of Dental Research 91 (11) : 1049-1054. ScholarBank@NUS Repository. https://doi.org/10.1177/0022034512459053
Abstract: Crosslinking is considered a possible approach to increasing the mechanical and structural stability and biodegradation resistance of the dentin collagen matrix. The aim of this study was to investigate the mechanical and chemical variations and collagen degradation resistance associated with crosslinking of the dentin collagen matrix with UVA-activated riboflavin. Dentin collagen matrix specimens were treated with 0.1 and 1% riboflavin for 2 min and photo-activated with 7 mW/cm2 UVA (368 nm) for 2 min. The structural change of the dentin collagen network with collagenase exposure was investigated by AFM and SEM at different time-points. The variations in surface/bulk mechanical properties and biodegradation resistance were characterized by nano-indentation, conventional mechanical testing, and hydroxyproline liberation at different time-points. Chemical changes associated with riboflavin/collagen-matrix interaction were analyzed by micro-Raman spectroscopy. UVA-activated riboflavin increased the mechanical properties, mechanical stability, and biodegradation resistance of the dentin collagen matrix. Higher collagen-network structural resistance against collagenolytic challenges was found with crosslinking. micro-Raman spectroscopy showed a strong dependency, in both intensity and wave-number, of certain Raman bands (1242-1667 cm-1) with crosslinking indicating the collagen/riboflavin interactions. UVA-activated riboflavin (1%) more efficiently crosslinked the dentin collagen matrix within a relatively clinically acceptable time-frame compared with 0.1% riboflavin. © 2012 International & American Associations for Dental Research.
Source Title: Journal of Dental Research
URI: http://scholarbank.nus.edu.sg/handle/10635/47136
ISSN: 00220345
DOI: 10.1177/0022034512459053
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