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|Title:||Effects of elevated temperature in vivo on the maturational and developmental competence of porcine germinal vesicle stage oocytes|
In Vitro Maturation
|Source:||Tong, G.Q.,Heng, B.C.,Chen, N.Q.,Yip, W.Y.,Ng, S.C. (2004). Effects of elevated temperature in vivo on the maturational and developmental competence of porcine germinal vesicle stage oocytes. Journal of Animal Science 82 (11) : 3175-3180. ScholarBank@NUS Repository.|
|Abstract:||Postslaughter processing of sow carcasses results in the ovaries being exposed to temperatures of 41.3 to 42.1°C within a 30-min time frame. This study investigated whether the maturational and developmental competence of the recovered germinal vesicle stage oocytes could be compromised by post-slaughter processing. The results showed that the in vitro maturation rates of GV stage oocytes exposed to elevated temperature did not significantly differ from the corresponding controls (74.1 vs. 75.8%). Immunocytochemical staining revealed that elevated temperature did not adversely affect metaphase II spindle formation but resulted in extensive disruption of oocyte cytoskeletal organization. This, in turn, had a detrimental effect on parthenogenetic development compared with the corresponding nonheat-treated controls (cleavage rate = 27.7 vs. 65.3%, P < 0.01; blastulation rate = 6.7 vs. 20.6%, P < 0.01). Hence, transient exposure to elevated temperature during slaughter did not have any detrimental effects on nuclear maturation per se, but it did result in extensive cytoskeletal damage, which in turn drastically decreased the developmental competence. © 2004 American Society of Animal Science. All rights reserved.|
|Source Title:||Journal of Animal Science|
|Appears in Collections:||Staff Publications|
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