Please use this identifier to cite or link to this item: https://doi.org/10.1007/s10815-006-9083-8
Title: Exposure of mouse cumulus cell nuclei to porcine ooplasmic extract eliminates TATA box protein binding to chromatin, but has no effect on DNA methylation
Authors: Tong, G.Q.
Heng, B.C. 
Ng, S.C.
Keywords: Methylation
Nuclear reprogramming
Ooplasm
Porcine
TATA box
Issue Date: 2006
Source: Tong, G.Q.,Heng, B.C.,Ng, S.C. (2006). Exposure of mouse cumulus cell nuclei to porcine ooplasmic extract eliminates TATA box protein binding to chromatin, but has no effect on DNA methylation. Journal of Assisted Reproduction and Genetics 23 (11-12) : 413-419. ScholarBank@NUS Repository. https://doi.org/10.1007/s10815-006-9083-8
Abstract: Purpose: The low cloning efficiency with SCNT is due to incomplete or partial reprogramming of the donor somatic cell nuclei after microinjection into the enucleated oocyte. A possible solution may be to initiate nuclear reprogramming prior to SCNT. Methods: Pre-exposure of donor somatic cell nuclei to a novel porcine ooplasmic extract prior to microinjection could possibly extend the duration of exposure to ooplamic nuclear reprogramming factors. The effects of the porcine ooplamic extract on two major markers of nuclear preprogramming: (1) TATA box protein binding to chromation and (2) DNA methylation was investigated. Results: The results showed that pre-exposure of mouse cumulus cell nuclei to porcine ooplamic extract drastically reduced TATA box protein binding to chromatin, but had no effect on DNA methylation. Conclusions: Pre-exposure to the porcine ooplasmic extract had some limited effects on nuclear reprogramming. Whether this can lead to enhanced cloning efficiency needs to be further investigated. © 2006 Springer Science+Business Media, LLC.
Source Title: Journal of Assisted Reproduction and Genetics
URI: http://scholarbank.nus.edu.sg/handle/10635/47078
ISSN: 10580468
DOI: 10.1007/s10815-006-9083-8
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

4
checked on Dec 12, 2017

Page view(s)

64
checked on Dec 7, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.