Please use this identifier to cite or link to this item: https://doi.org/10.1017/S0967199404002461
Title: Investigations of oocyte in vitro maturation within a mouse model
Authors: Chin, A.H.B. 
Chye, N.S.
Keywords: Follicular fluid
Granulosa
In vitro maturation
Murine
Oocyte
Serum
Issue Date: 2004
Source: Chin, A.H.B., Chye, N.S. (2004). Investigations of oocyte in vitro maturation within a mouse model. Zygote 12 (1) : 1-18. ScholarBank@NUS Repository. https://doi.org/10.1017/S0967199404002461
Abstract: This study attempted to develop a 'less meiotically competent' murine model for oocyte in vitro maturation (IVM), which could more readily be extrapolated to human clinical assisted reproduction. Oocyte meiotic competence was drastically reduced upon shortening the standard duration of in vivo gonadotrophin stimulation from 48 h to 24 h, and by selecting only naked or partially naked germinal vesicle oocytes, instead of fully cumulus enclosed oocyte complexes. With such a less meiotically competent model, only porcine granulosa coculture significantly enhanced the oocyte maturation rate in vitro, whereas no significant enhancement was observed with macaque and murine granulosa coculture. Increased serum concentrations and the supplementation of gonadotrophins, follicular fluid and extracellular matrix gel within the culture medium did not enhance IVM under either cell-free or coculture conditions. Culture medium conditioned by porcine granulosa also enhanced the maturation rate, and this beneficial effect was not diminished upon freeze-thawing. Enhanced IVM in the presence of porcine granulosa coculture did not, however, translate into improved developmental competence, as assessed by in vitro fertilization and embryo culture to the blastocyst stage.
Source Title: Zygote
URI: http://scholarbank.nus.edu.sg/handle/10635/47066
ISSN: 09671994
DOI: 10.1017/S0967199404002461
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