Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.joen.2007.01.018
Title: Advanced Noninvasive Light-activated Disinfection: Assessment of Cytotoxicity on Fibroblast Versus Antimicrobial Activity Against Enterococcus faecalis
Authors: George, S.
Kishen, A. 
Keywords: Cytotoxicity
disinfection
Enterococcus faecalis
fibroblast
light
sodium hypochlorite
Issue Date: 2007
Citation: George, S., Kishen, A. (2007). Advanced Noninvasive Light-activated Disinfection: Assessment of Cytotoxicity on Fibroblast Versus Antimicrobial Activity Against Enterococcus faecalis. Journal of Endodontics 33 (5) : 599-602. ScholarBank@NUS Repository. https://doi.org/10.1016/j.joen.2007.01.018
Abstract: Recent interest in light-activated disinfection demands insight on the selectivity towards bacterial cells compared with mammalian cells. This study was aimed to evaluate the cytotoxicity and selectivity of an advanced noninvasive light-activated disinfection (ANILAD) developed in our laboratory. The extent of cytotoxic effect of methylene blue activated by visible light of wavelength 664 nm was tested and compared with sodium hypochlorite (NaOCl) under in vitro and ex vivo conditions on fibroblast L929 cells. Simultaneous evaluation of cytotoxicity and antibacterial effect was also conducted to study the specificity of light-activated therapy (LAT) toward prokaryotic cells (Enterococcus faecalis). The cytotoxicity was evaluated by 3-(4, 5-dimethylthiazol-2- yl)-2, 5-diphenyltetrazolium bromide assay and trypan blue viability test, whereas colony-forming units were determined to evaluate bacterial viability. Data from both in vitro and ex vivo experiments showed that cytotoxicity was significantly less in LAT compared with NaOCl (p < 0.001). E faecalis cells were killed at a faster rate than fibroblasts. An irradiation dose producing 97.7% bacterial killing showed only 30% fibroblast dysfunction. This study indicated that ANILAD produced an insignificant effect on mammalian cells. © 2007 American Association of Endodontists.
Source Title: Journal of Endodontics
URI: http://scholarbank.nus.edu.sg/handle/10635/46808
ISSN: 00992399
DOI: 10.1016/j.joen.2007.01.018
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

45
checked on Sep 21, 2018

WEB OF SCIENCETM
Citations

45
checked on Sep 11, 2018

Page view(s)

60
checked on Jul 13, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.