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Alternative Title
Abstract
Ultrafast lasers have found increasing use in scanning optical microscopy due to their very high peak power in generating multiphoton excitations. A mode-locked Ti:sapphire laser is often employed for such purposes. Together with a synchronously pumped optical parametric oscillator (OPO), the spectral range available can be extended to 1050-1300 nm. This broader range available greatly facilitates the excitation of second harmonic generation (SHG) and third harmonic generation (THG) due to better satisfaction of phase matching condition that is achieved with a longer excitation wavelength. Dental sections are then investigated with the contrasts from harmonic generation.
Keywords
Laser scanning microscopy, Optical parametric oscillator, Second harmonic generation, Third harmonic generation, Two-photon excitation
Source Title
Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Publisher
Series/Report No.
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Date
2005
DOI
10.1117/12.577285
Type
Conference Paper