Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/46603
Title: Localization of glutamate receptor subunits of the α-amino-3-hydroxy- 5-methyl-4-isoxazolepropionate (AMPA) type in the pancreas of newborn guinea pigs
Authors: Liu, H.-P. 
Tay, S.S.-W.
Leong, S.-K.
Keywords: α-Amino-3-hydroxy-5-methyl-4- isoxazolepropionate
diaphorase
Ganglia
Glutamate
Guinea pig
Islets
NADPH
Receptor
Issue Date: 1997
Source: Liu, H.-P.,Tay, S.S.-W.,Leong, S.-K. (1997). Localization of glutamate receptor subunits of the α-amino-3-hydroxy- 5-methyl-4-isoxazolepropionate (AMPA) type in the pancreas of newborn guinea pigs. Pancreas 14 (4) : 360-368. ScholarBank@NUS Repository.
Abstract: Glutamate and related molecules are the major excitatory neurotransmitters in the central nervous system, and their receptors have been localized therein. Little is, however, known about them in the peripheral nervous system. The present study investigated the localization of glutamate receptor subunits of the α-amino-3-hydroxy-5-methyl-4- isoxazolepropionate (AMPA) type (GluR1, GluR2-3, and GluR4) in the pancreas of newborn guinea pigs. With a double-labeling method of immunofluorescence and immuno-tetrahydrochloride reaction, GluR1 and GluR4 immunoreactivities were localized mostly in the insulin-secreting cells in the central mass of the islet, and GluR2-3 immunoreactivity in the peripheral rim of the islet, which consists mainly of non-insulin-secreting islet cells. With a double- labeling method employing immuno-fluorescence and NADPH-diaphorase (NADPH-d) histochemistry, GluR2-3 and GluR4 immunoreactivities were localized in most of the NADPH-d-positive pancreatic ganglion cells. None of the NADPH-d- positive ganglion cells showed GluR1 immunoreactivity. In fact, GluR1 immuno- reactivity was not detected in any of the pancreatic ganglion cells. The results indicate that glutamate is likely to exert its effects on the pancreas by activating different AMPA receptor subunits located in endocrine cells and intrapancreatic ganglia.
Source Title: Pancreas
URI: http://scholarbank.nus.edu.sg/handle/10635/46603
ISSN: 08853177
Appears in Collections:Staff Publications

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